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• What are the three most important analytical techniques used to characterize a metabolome? NMR; GC/MS; LC/MS
• Please define “metabolic fingerprinting”. Classification of samples in regards to biological relevance and following identification of single biomarkers
• Please define “metabolite profiling”. Quantitative and qualitative analysis of selected biomarkers
• What is the most reliable approach for MS-based compound identification? LC/MS-MS and comparison with a library ?
• Please summarize advantages and disadvantages of using LC/MS for metabolomics. Pros: superb sensitivityFlexiblemost organic, some inorganic compoundsminimal sample size neededMetabolite imaging (MALDI)Cons: sample not recoverableNot very quantitativeExpensiveslow poor seperation resolution and reproducibilty (vs GC)

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