5
Onko (Subject) / 9. Klocker (Lesson)
- What can be analyzed with a reporter gene assay? aus Praktikumsskript. the activity of: transcription factors. transciptional co-activators and co-repressor, other proteins of the transcription machinery, DNA elements of gene transcription (binding sites for transcription factors and proteins if the transcription machinery; test transcription factor inhibitors/activators
- Domains and structural motives of the androgen receptor protein? DNA binding site has two zincfingers ??? Domains: DNA bdg site; Ligand binding domain; N-Terminal regulatory domain; C-terminal regulatory domain highly conserved nuclear receptor ligand-binding domain signature motif
- Which regulation cascade controls the level of androgens in the human? hypothalamus (GnRH) - pituitary gland (ACTH) - adrenal gland (DHEA->T->DHT) Hypothalamus (GnRH) -> anterior pituitary (LH, FSH - gonadotropins) -> LH induces testosterone production in the Leydig cells of the testes, FSH induces spermatogenesis and inhibin production -> inhibin gives a negative feedback to the pituitary gland (no more FHS and LH released) Testosterone -> DHT in the periphery by 5 alpha reductase. Adreanal gland only responsible for a small amount of androgen production!
- Treatment options and treatment sequence for prostate cancer? first line treatment: moitoring, radical surgery, radiationsecond line treatment: radiation, androgen hormone ablation therapythird line tratment: 3rd generation antiandrogens, CYP17A inhibitor, Chemotherapy Docetaxel and Carbotaxel, AlphaRadin (Radium 223 chloride), Immunotherapy
- What is PSA and how is it used? A glycoprotein, protease of the kallikrein family, appears in the blood circulation only when prostate tissue architecture is disturbed -> prostate disease
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