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Bacteriologie (Subject) / Bacterio III und IV (Lesson)
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- Staphylococcus (key points) • Gram-positive cocci in clusters resembling bunches of grapes • Grow on non-enriched media • Moderately-sized white or golden colonies • Colonies of S. aureus and S. pseudintermedius produce double haemolysis • Facultative anaerobs • Non-motile • Catalase-positive • Coagulase production correlates with pathogenicity • Comparatively stable in the environment • Cause pyogenic infections
- Streptococcus • Gram-positive cocci in chains • Fastidious, requiring enriched media (BA) • Small usually haemolytic, translucent colonies • Facultative anaerobs • Usually non-motile • Catalase-negative • Commensals on mucous membranes • Susceptible to desiccation • Cause pyogenic infections
- Erysipleothrix rhusiopathiae - alimentary tract, mud, soil, slime of fishes, water - 1-3 thin rods, chains, filament(Rtyp) -culture: nutrient agar, blood agar: some staoms alpha haem. -catalase + oxidase: - - fermentative -neuaminidase -hyaluronidase -2 common protein antigens - good restiance ,halotolerance, soil -facultativ pathogenic
- Pathogenicity of Erysipelothrix rhusiopathiae swine: erysipleas (Rotlauf) sheep: wound infection birds: septicaemia humans:wound infechtion
- Erysipleothrix tonisillarum in tonsills of swine endocarditis in dogs serotyp 7 and further 6 no biochem difference to E.rhusi
- Habitat und Morphology Listeria -soil, sewage,plants,silage, gut,food -1-2micrometer thick rods -flagella(22grad) -gram pos
- Culture L. monoscytogentes simple beta-haemolysis 4-45 Grad ph: 5,6-9,6 selective: cattle serum -tyrpaflavin-nalixicisd acid selective enirochment: - cold 4 grad and Utube
- Biochem. L myocytogentes catalase + oxidase - fermentativ
- Antigens L. myocytogentes cell wall polysaccarides flagellar protein 16 serotyps: most common-> 1/2a,4b
- Restistance and Pathogen. of L.myocytogentes - Restistance : months in soil, propagation(Übertragung) - facutative pathogenic - virulence: haemolysis llipolysis monocytosis factor cell walls lipoids
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- Pathogenicity L.myocytogentes sheep: mainly CNS signs cattle: mainly abortion rabbit: septiceamia humans:abortion, newborn septicaemia, enchepathlitis birds: septicaemia
- species Listeria L.monocytogentes L.ivanovii (better haemo.,serotypes 5) intra celluar agents saprophytes
- Habtiat and Morpho and culture of Corynebacterium - in mucosus membrans, skin, soil, milk -polymorphism -metachromatic granules -gram pos slightly acid-fast - Culture: blood agar, haemolysis
- Biochem, Antigens, Resistance of Corynebacterium -catalase +, oxidase-, fermentative -toxin: C.diphteriae, C. pseudotuberculosis Antigens:complex Restisance:averge
- Species : C diphtheria + pseudozuberculosis C.diphtheria: exotoxin phage C. pseudotub.:polymorph., fimbria, haemoly - exotoxin:phospholipase D -nitate neg: sheep,goat: abortion, arthritis -nitrate pos:horse: equine ulcerative lymphadenitis
- Species : C diphtheria + pseudotuberculosis C.diphtheria: exotoxin phage C. pseudotub.:polymorph., fimbria, haemoly - exotoxin:phospholipase D -nitate neg: sheep,goat: abortion, arthritis -nitrate pos:horse: equine ulcerative lymphadenitis
- species: C. renale, C. pilosum,C. cystitidis, C.bovis, C. kutscheri C.renale: fimbria, cattle cysitits and pyelitis C.pilosum:cattle cyisitis, pyelonphritis C.cystitidis: cattle haemorrhagic cyisitis C. bovis: udder, milk,, mastitis, abortion C.kutscheri: rodents
- Rodococcus equi: Habitat, Morph,Staining culture - in soil, plant, gut -coccux rod cycle, capsule -gram pos., some acid fast - simple culture, capsule, pigment(pink)
- Pathogenicity of rhodococcus intracelluar foal pneumonia horse gut mesentreric lymph nodes, abscecces(cattle, swine) human pneumonia(AIDS)
- Renibacterium salmoninarium in water, fish 1-1,5 micrometer rods,short chains gram positive aerobic, 15-18 grad,fastioius obligate pathogenic, intracelluar, salomon,trouts: abscesses in kidney and other organs