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Virologie (Fach) / Praktikum 2 (Lektion)
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- In case of haemagglutination, what is the red blood cell agglutinated by? By the virus
- What is the case of haemadsorption? The virus induces proteins on cell, attaching red blood cells
- If the virus loses its inefectivity after chloroform treatment, then it is..? An enveloped virus
- If the virus is treated will chloroform and continues to grow in the cell culture it is? Non-enveloped
- If the virus is colored greenish/yellowish after acridinorange treatment it is? Double stranded
- If a virus does not cause CPE..? It cannot be investigated in cell cultures
- The haemagglutination titer is..? The last dilution where haemagglutination is observed
- Acridine orange turns.. reddish/orange with nucleic acids
- We can observe synctytium in..? Both stained and non stained cells
- Inclusion bodies are formed by? Both DNA and RNA viruses
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- In immunoperoxidase test..? The peroxide is added, attached to the antibody or to the virus
- What kind of viruses is stopped with deoxyuridine? DNA viruses only
- Plaque is..? CPE in the monolayer
- Lumpy cell nucleus are a result of? Chromatin conglomerulate
- What is an HA unit? The last hemagglutination titer
- Why do we titrate orange? Single/double threaded nucleic acid (ssNA/dsNA)
- What do we use for immune peroxide assay? virus-specific antibody labelled with peroxidase enzyme
- What kind of viruses can attract erythrocytes to cell membrane? Orthomyxoviridae, Paramyxoviridae, Coronaviridae, Parvoviridae
- Does CPE have diagnostic value? Sometimes, but not pathogenic.