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Virology (MD PhD) (Fach) / Trkola (Lektion)
- Betaherpesvirinae - HCMV, HHV-6 - slow replication cycle and slow spread in culture - latency in secretory glands, lymphoreticular cells, kidney, ... - infected cells become large (cytomegalia)
- Gammaherpesvirinae - EBV, HHV8 - replicate in either B- or T-cells - latency frequently in lymphoid tissues
- Modified Koch‘s postulates for viruses 1. Isolation of virus from infected host organism 2. Cultivation in host cells 3. Demonstration of filterability 4. Production of comparable disease with isolated virus in original or related host species5. Re-isolation of virus from inocculated host 6. Demonstration of specific immune response to virus
- T-20 = Enfuvirtide (INN) - HIV fusion inhibitor - binds to gp41 preventing the creation of an entry pore
- Accessory proteins HIV - tat (trans-activator of transcription): Required for transcription. Activates transcription by binding to TAR. - rev (regulator of virion): allows transport of unspliced mRNA out of the nucleus - nef: reduces MHCI expression - vif (viral infectivity factor): downregulates APOBEC - vpu (viral protein u): reduces expression of Tetherin - vpr (viral protein r): induces apoptosis
- Structural proteins HIV - gag: Matrix, capsid - pol: Protease, reverse transcriptase, integrase - env: gp120/41
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