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IMCH (Fach) / Techniken (Lektion)

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• Agardiffusion - Test ? single diffusion test: radial immunodiffusion double diffusion test: Ouchterlony   prinzipe: precipitate line
• what is radial immunodiffusionstest? Antibody in Gel Antigen is set on it --> radial preciptate line   comparison to standard graph --> concentration of AG
• what is Laurell immuno-electrophoresis? Rocket   Gel with AB AG --> Rockets  
• crossed immuno electrophoresis 2D (Clark Freeman) 1. Dim: nach isoelektrischem Punkt separation of protein mixture   2. Dim: nach Molekulargewicht --> Rockets  
• Variants of crossed Immuno-electrophoresis fused rocket tandem crossed line-immuno-electrophoresis crossed line-immuno-electrophoresis  
• Western blotting: 1. mixture of proteins ---> separated bei SDS-gel electrophoresis 2. transfer of prot. to membrane by vertical electric field (copy) 3. add substrate to activate color reaction  
• western blotting: 1. mixture of proteins separated by sds-gel-electroph. 2. transfer to membrane by vertical electric field (copy) 3. add substrate to activate color reaction
• what means blotting? transfer of macromolecules from one material to another (gel -> membrane)
• Types of Blotting: Southern Blotting: DNA Northern Blotting: RNA Western Blotting: Proteins  

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